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Biochemicals> | … “HOAT + EDAN solution” was a solution of the secondary activating reagent 7-aza-1- hydroxybenzotriazole (HOAT) (Genscript Corporation, Piscataway, USA) at 6 g L −1 and the derivatizing reagent N-(1-naphthyl)ethylenediamine (EDAN) (Sigma-Aldrich) at 2 g L −1 , prepared … | Get A Quote |
In this paper, a new thin-layer chromatography (TLC) method for the determination of individual volatile fatty acids (VFAs) is presented. The experimental procedure is based on the derivatization of VFAs with activating agents and a naphthalene-based amine to form an amide derivative, followed by fast TLC separation of these VFA derivatives and visualization with phosphomolybdic acid at room temperature. Quantification is then performed by densitometric measurements using free software after taking TLC pictures in a home-made black box for homogeneous and reproducible lighting of the plates. Optimization of the experimental conditions enables fast determination (20 minutes for 3 samples) of VFAs with detection ... More